Haedicke, W. and Wolf, H. and Ehret, W. and Reischl, U. (1996) Specific and sensitive two-step polymerase chain reaction assay for the detection of Salmonella species. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 15 (7). pp. 603-607. ISSN 0934-9723, 1435-4373
Full text not available from this repository.Abstract
A polymerase chain reaction (PCR) assay was applied for the selective amplification of a characteristic sequence within a Salmonella-specific chromosomal fragment, A two-temperature PCR cycle enhanced both the speed and overall sensitivity of the amplification procedure, Twenty-one well-characterized Salmonella strains and a number of non-Salmonella strains were tested, With the exception of the rarely isolated Salmonella arizonae strain, the PCR-based approach enabled the specific identification of Salmonella with a detection limit of 10(3) organisms, In combination with a nested PCR assay, as few as ten organisms were detectable, Specificity was demonstrated as no distinct amplification products were detectable with any of the tested non-Salmonella strains, With a pre-enrichment step using paramagnetic anti-Salmonella beads, an increase in sensitivity was observed in the case of clinical samples while the amplification process was not influenced.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IMMUNOMAGNETIC SEPARATION; LISTERIA-MONOCYTOGENES; RAPID DETECTION |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Jul 2023 05:22 |
| Last Modified: | 05 Jul 2023 05:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51612 |
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