P840-reaction centers from Chlorobium tepidum - Quinone analysis and functional reconstitution into lipid vesicles

Frankenberg, Nicole and Hager-Braun, Christine and Feiler, Ute and Fuhrmann, Markus and Rogl, Hans and Schneebauer, Nikolaus and Nelson, Nathan and Hauska, Günter (1996) P840-reaction centers from Chlorobium tepidum - Quinone analysis and functional reconstitution into lipid vesicles. PHOTOCHEMISTRY AND PHOTOBIOLOGY, 64 (1). pp. 14-19. ISSN 0031-8655, 1751-1097

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Abstract

Membranes of Chlorobium tepidum contain about 35, 45 and 2-10 molecules of menaquinone-7, chlorobium quinone (1'-oxo-menaquinone-7) and of the polar menaquinone (probably 1'-OH-menaquinone-7) per reaction center, respectively. None of these quinones was retained during the isolation of P840-reaction centers beyond the detection limit of about 0.2 quinones per reaction center, neither in the core complex nor in functionally intact reaction center preparations. The latter is shown to catalyze the formation of an electrochemical proton gradient in the presence of ascorbate and phenazinium methosulfate, when it is incorporated into lipid vesicles.

Item Type: Article
Uncontrolled Keywords: PHOTOSYNTHETIC REACTION CENTER; GREEN SULFUR BACTERIA; PHOTOSYSTEM-I; HELIOBACTERIUM-CHLORUM; LIMICOLA; PHOTOPHOSPHORYLATION; CHLOROPLASTS; MEMBRANES
Subjects: 500 Science > 580 Botanical sciences
Divisions: Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Alumni or Retired > Prof. Dr. Günter Hauska
Depositing User: Dr. Gernot Deinzer
Date Deposited: 05 Jul 2023 06:09
Last Modified: 05 Jul 2023 06:09
URI: https://pred.uni-regensburg.de/id/eprint/51627

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