Light-induced charge separation between plastocyanin and the iron-sulfur clusters F-A and F-B in the complex of plastocyanin and photosystem I

Hippler, Michael and Riedel, Astrid and Schroer, Uwe and Nitschke, Wolfgang and Haehnel, Wolfgang (1996) Light-induced charge separation between plastocyanin and the iron-sulfur clusters F-A and F-B in the complex of plastocyanin and photosystem I. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 330 (2). pp. 414-418. ISSN 0003-9861, 1096-0384

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Abstract

The light-induced electron transfer in a crosslinked complex between plastocyanin and photosystem I from spinach was studied by EPR at low temperature. Electron donation from reduced plastocyanin to P700(+) was observed under illumination above a temperature of about 160 K, resulting in a second charge separation and an electron transfer from rereduced P700 to the terminal electron acceptors F-A/F-B The charge-separated state Pc(ox)P700(+)[F-A/F-B](2-) was found to be stable at 15 K, Implications of these results for the kinetic constants of the donation reaction and the backtransfer of electrons from reduced accepters as well as for the structural models of the terminal accepters are discussed. (C) 1996 Academic Press, Inc.

Item Type: Article
Uncontrolled Keywords: SITE-DIRECTED CONVERSION; CHEMICAL CROSS-LINKING; ELECTRON-TRANSFER; ABSORPTION-SPECTROSCOPY; EXTINCTION COEFFICIENTS; BINDS PLASTOCYANIN; REACTION CENTERS; IDENTIFICATION; SUBUNIT; POLYPEPTIDE; photosystem I; plastocyanin; iron-sulfur clusters F-A and F-B; P700; electron transfer; EPR; spinach
Subjects: 500 Science > 570 Life sciences
Divisions: Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie
Depositing User: Dr. Gernot Deinzer
Date Deposited: 05 Jul 2023 06:40
Last Modified: 05 Jul 2023 06:40
URI: https://pred.uni-regensburg.de/id/eprint/51642

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