Arndt, H and Bolanowski, MA and Granger, DN (1996) Role of interleukin 8 on leucocyte-endothelial cell adhesion in intestinal inflammation. GUT, 38 (6). pp. 911-915. ISSN 0017-5749,
Full text not available from this repository.Abstract
Background-An important action of interleukin 8 (IL8) is stimulation of granulocytes. The object of this study was to assess the contribution of IL8 to the leucocyte-endothelial cell interactions associated with intestinal inflammation in the rat. Methods Two indomethacin injections (48 and 24 hours prior to the experiments) induced a longlasting ileitis in rats. The number of adherent and emigrated leucocytes, leucocyte rolling velocity, and shear rate were monitored in normal and inflamed mesenteric postcapillary venules. Some animals received a monoclonal antibody (MAb) against IL8 or CD11b/CD18 at 24 and 12 hours prior to the experiment. Results-Indomethacin elicited a sevenfold increase in leucocyte adherence and a 5.4-fold increase in leucocyte emigration, while leucocyte rolling velocity was reduced by nearly 80%. The indomethacin induced increases in leucocyte adherence and emigration were significantly reduced (by 57% and 67%, respectively) while leucocyte rolling velocity was increased (to 63% of control) by the IL8-specific MAb. The level of inhibition seen with the IL8 MAb was similar to that associated with administration of a MAb directed against the leucocyte adhesion molecule CD11b/CD18. Conclusions-IL8 contributes to the leucocyte-endothelial cell interactions elicited in mesenteric venules by indomethacin.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLONY-STIMULATING FACTOR; LEUKOCYTE ADHESION; MESSENGER-RNA; ULCERATIVE-COLITIS; SURFACE EXPRESSION; HUMAN NEUTROPHILS; MUCOSAL LESIONS; BOWEL-DISEASE; CYTOKINE; REPERFUSION; indomethacin; microcirculation; inflammatory bowel disease |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51666 |
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