Bohmann, E. -M. and Fehn, U. and Holler, B. and Weber, D. and Holler, E. and Herr, W. and Hoffmann, P. and Edinger, M. and Wolff, D. (2017) Altered immune reconstitution of B and T cells precedes the onset of clinical symptoms of chronic graft-versus-host disease and is influenced by the type of onset. ANNALS OF HEMATOLOGY, 96 (2). pp. 299-310. ISSN 0939-5555, 1432-0584
Full text not available from this repository. (Request a copy)Abstract
We analyzed lymphocyte subpopulations and cytokines 3 months after allogeneic hematopoietic stem cell transplantation aiming to identify predictive cellular and serum markers for chronic graft-versus-host disease (cGVHD). Samples of 49 patients (pts) (no cGVHD (n = 14), subsequent quiescent onset (n = 16), de novo onset of cGVHD (n = 19)) were analyzed in the absence of active GVHD by flow cytometry and enzyme-linked immunosorbent assay. All mean absolute cell counts are presented as cells per microliter; relative cell counts are presented as percentage of lymphocytes. Pts with subsequent de novo cGVHD had significantly higher relative and absolute counts of CD4+ T cells including higher absolute counts of CD4+ memory T cells (22.36%; 206.55/mu l; 136/mu l, respectively) compared to pts with subsequent quiescent onset of cGVHD (12.41%; 83.42/mu l; 54.3/mu l) and pts without cGVHD (10.55%) with regard to relative counts of CD4+ T cells. Similarly, significantly more relative and absolute regulatory T cell numbers (CD4+FOXP3+) were detected in pts with de novo onset of cGVHD (3.08% and 24.63/mu l) compared to those in pts without (1.25% and 9.06/mu l) or with quiescent onset of cGVHD (1.15% and 6.91/mu l). Finally, relative B cell counts, including na < ve and memory B cells, were also significantly decreased in pts developing quiescent cGVHD (0.85, 0.73, 0.12% resp.) when compared to pts with de novo onset (5.61, 5.24, 0.38%). The results demonstrate that alterations in immune reconstitution are already present before onset of clinical symptoms and differ between de novo and quiescent onset of disease.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BONE-MARROW-TRANSPLANTATION; CHRONIC GVHD; RISK; ASSOCIATION; DEFICIENCY; DIAGNOSIS; RESPONSES; CORRELATE; RECOVERY; SURVIVAL; Allogeneic hematopoietic stem cell transplantation; Graft-versus-host disease; Bone marrow transplantation; BAFF; Immature B cells |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Regensburger Centrum für Interventionelle Immunologie (RCI) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:01 |
| Last Modified: | 18 Feb 2019 11:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/517 |
Actions (login required)
 |
View Item |
