Absorption and fluorescence spectroscopic investigation of indocyanine green

Philip, Reji and Penzkofer, Alfons and Bäumler, Wolfgang and Szeimies, Rolf-Markus and Abels, Christoph (1996) Absorption and fluorescence spectroscopic investigation of indocyanine green. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 96 (1-3). pp. 137-148. ISSN 1010-6030

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Abstract

Absorption spectra, fluorescence quantum distributions, fluorescence quantum yields and degrees of fluorescence polarization vs, dye concentration were determined for indocyanine green in methanol, water and aqueous albumin solution. The monomer fluorescence quantum yield is limited to a few per cent by internal conversion. In water, dimerization starts at low concentrations (approximately 3 x 10(-8) mol dm(-3)) and lowers the fluorescence quantum yield. The strong affinity of the dye to albumin shifts the onset of dimerization to higher concentrations. in methanol, dimerization is weak and closely spaced pair formation dominates at high concentrations. Dye adsorption to albumin and dye aggregation in the solvents were analysed experimentally and theoretically.

Item Type: Article
Uncontrolled Keywords: RHODAMINE 6G SOLUTIONS; DYE; BEHAVIOR; PHARMACOKINETICS; DYSFUNCTION; GENERATION; ENZYME; SERUM; absorption; fluorescence; indocyanine green
Subjects: 500 Science > 530 Physics
600 Technology > 610 Medical sciences Medicine
Divisions: Medicine > Lehrstuhl für Dermatologie und Venerologie
Physics > Institute of Experimental and Applied Physics > Alumni or Retired Professors > Group Alfons Penzkofer
Depositing User: Dr. Gernot Deinzer
Date Deposited: 06 Jul 2023 06:18
Last Modified: 06 Jul 2023 06:18
URI: https://pred.uni-regensburg.de/id/eprint/51700

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