Schmalz, G and Garhammer, P and Schweiki, H (1996) A commercially available cell culture device modified for dentin barrier tests. JOURNAL OF ENDODONTICS, 22 (5). pp. 249-252. ISSN 0099-2399,
Full text not available from this repository.Abstract
The suitability of a dentin barrier test based on a commercially available cell culture chamber was evaluated by testing the cytotoxicity of dental cements, The two chambers of the culture device as produced are separated by a membrane. This was replaced by a bovine dentin disk (500 mu m thick), Mouse fibroblasts were grown on the ''pulpal'' side of the dentin for 24 h; test materials were then placed into the ''cavity'' side of the upper chamber, The number of viable cells was determined after 24 h, After exposure to zinc phosphate cement at a powder/liquid ratio of 2:1, similar to 100% of cells survived, A ratio of 1:1 yielded 81% survival, Only 24% and 28% of the cells survived after exposure to Ketac Fil and Ketac Silver, respectively, The light-curing glass ionomer cement (Vitrebond) and zinc oxide-eugenol killed all cells, These results agree with those obtained from a previous study, wherein the dentin barrier test device was constructed in our laboratory.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CYTO-TOXICITY; RESTORATIVE MATERIALS; GLASS IONOMER; PULP; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51739 |
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