Bocker, T and Schlegel, J and Kullmann, F and Stumm, G and Zirngibl, H and Epplen, JT and Ruschoff, J (1996) Genomic instability in colorectal carcinomas: Comparison of different evaluation methods and their biological significance. JOURNAL OF PATHOLOGY, 179 (1). pp. 15-19. ISSN 0022-3417,
Full text not available from this repository.Abstract
In order to demonstrate the relationship between microsatellite instability and other types of genomic instability a series of 56 sporadic colorectal carcinomas was investigated by flow cytometrical ploidy analysis, oligonucleotide fingerprinting, and microsatellite polymerase chain reaction (PCR). Stabilization of the p53 gene product was analysed by immunohistochemistry and proliferative activity was determined flow cytometrically and by silver staining of nucleolar organizer regions (AgNORs). Of the 56 carcinomas, 11 (19 per cent) exhibited microsatellite instability; 33 of the cases were aneuploid (59 per cent) and 29 (52 per cent) showed alterations of the oligonucleotide fingerprints, There was a significant correlation of microsatellite instability with localization of these rumours proximal to the splenic flexure, diploid DNA content, and less frequent p53 stabilization. A solid growth pattern, mucinous differentiation, and a Crohn's-like lymphoid infiltrate were also characteristic for those rumours. The results demonstrate for the first time a significantly lower proliferative activity in tumours with microsatellite instability, Data obtained from DNA flow cytometry or from oligonucleotide fingerprinting did not correlate with such tumour characteristics. It is proposed that the use of microsatellite PCR facilitates specifically the detection of a group of colorectal cancers which may differ in pathogenesis and perhaps prognosis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CANCER; DNA; colorectal carcinoma; genomic instability; microsatellite analysis; DNA fingerprinting; DNA ploidy |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51744 |
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