Brand, Korbinian and Page, Sharon and Rogler, Gerhard and Bartsch, Armin and Brandl, Richard and Knuechel, Ruth and Page, Michael and Kaltschmidt, Christian and Baeuerle, Patrick A. and Neumeier, Dieter (1996) Activated transcription factor nuclear factor-kappa B is present in the atherosclerotic lesion. JOURNAL OF CLINICAL INVESTIGATION, 97 (7). pp. 1715-1722. ISSN 0021-9738, 1558-8238
Full text not available from this repository.Abstract
Nuclear factor-kappa B (NF-kappa B)/Rel transcription factors play an important role in the inducible regulation of a variety of genes involved in the inflammatory and proliferative responses of cells. The present study was designed to elucidate the implication of NF-kappa B/Rel in the pathogenesis of atherosclerosis. Activation of the dimeric NF-kappa B complex is regulated at a posttranslational level and requires the release of the inhibitor protein I kappa B. The newly developed mAb alpha-p65mAb recognizes the I kappa B binding region on the p65 (RelA) DNA binding subunit and therefore selectively reacts with p65 in activated NF-kappa B. Using immunofluorescence and immunohistochemical techniques, activated NF-kappa B was detected in the fibrotic-thickened intima/media and atheromatous areas of the atherosclerotic lesion. Activation of NF-kappa B was identified in smooth muscle cells, macrophages, and endothelial cells. Little or no activated NF-kappa B was detected in vessels lacking atherosclerosis. Electrophoretic mobility shift assays and colocalization of activated NF-kappa B with NF-kappa B target gene expression suggest functional implications for this transcription factor in the atherosclerotic lesion. This study demonstrates the presence of activated NF-kappa B in human atherosclerotic tissue for the first time. Atherosclerosis, characterized by features of chronic inflammation and proliferative processes, may be a paradigm for the involvement of NF-kappa B/Rel in chronic inflammatory disease.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LOW-DENSITY-LIPOPROTEIN; ADHESION MOLECULE-1 VCAM-1; FACTOR GENE-EXPRESSION; THP-1 MONOCYTIC CELLS; FACTOR MESSENGER-RNA; SMOOTH-MUSCLE CELLS; TISSUE FACTOR; ENDOTHELIAL-CELLS; LIPOPOLYSACCHARIDE; ATHEROGENESIS; smooth muscle cells; macrophages; endothelial cells; p65; I kappa B |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Nov 2023 07:30 |
| Last Modified: | 02 Nov 2023 07:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51837 |
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