Minuth, Will W. and Kloth, S. and Aigner, J. and Sittinger, M. and Rockl, W. (1996) Approach to an organo-typical environment for cultured cells and tissues. BIOTECHNIQUES, 20 (3). pp. 498-501. ISSN 0736-6205
Full text not available from this repository.Abstract
If cells or tissues are taken our of an organ and put in culture, normally they lose morphological, physiological and biochemical features. This dedifferentiation process starts during the isolation procedure and continues during the whole culture period. Ir is caused by the stagnant liquid condition and the inadequate anchorage of cells at the bottom of tissue culture plasticware. The use of filters as basement membrane substitutes and the coating of culture-ware with extracellular matrix proteins improve the environmental factors for cultured cells but do not consider the paracrine influence of cytokines or the nutritional needs of individual cell types. To limit cellular dedifferentiation in culture, we constructed a new system, which adapts, as Snr as possible, cell and tissue cultures to an organo-typical environment. The system is based on a compatible cell carrier arrangement, which allows individual selection of supports for optimal cell anchorage and differentiation. The cell carriers are placed in a newly constructed container, which is permanently perfused with fresh culture medium. The system runs outside an incubator with simple laboratory tools; only a peristaltic pump, a warming table and pH-stabilized media are necessary. Without any subculturing, acute and chronic influences of drugs or the quality of medical implantation grafts can be studied over months.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLARITY |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie > Prof. Dr. Will Minuth |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Nov 2023 09:50 |
| Last Modified: | 02 Nov 2023 09:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51882 |
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