Molecular and microscopical studies in the Phellinus pini group

Fischer, Michael (1996) Molecular and microscopical studies in the Phellinus pini group. MYCOLOGIA, 88 (2). pp. 230-238. ISSN 0027-5514

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Abstract

Thirteen taxa of the Phellinus pini group from Europe (P. pini, P. chrysoloma), North America (P. piceinus, intersterility groups N-II ... N-VII), Morocco (P. cf. vorax), and Asia (intersterility groups As-I...As-III) were characterized by restriction-fragment analysis of rDNA and measurements of basidiospores and hymenial setae. A fragment of the rDNA, comprising the 5.8S rDNA, the ITSII region and parts of the 28S rDNA, was enzymatically amplified by PCR and digested with seven endonucleases. An identification of all taxa was not possible, and nine unique phenotypes were resolved among the thirteen taxa. Intersterility group N-IV was identical with N-VI and N-VII, and intersterility group N-V was identical with As-I and As-III. Based on the restriction-fragment data, a cluster analysis was performed through UFGMA. Phellinus cf. vorax and As-II were clearly separated from the remaining taxa. Phellinus piceinus turned out to be a sister taxon of P. chrysoloma. No apparent correlation was found between restriction-fragment data and geographic origin and host of the taxa studied. Both European taxa, P. pini and P. chrysoloma, were characterized by measurements of spores and setae; however, there was some overlap in measurements between these taxa. An unambiguous differentiation of non-European taxa was not possible.

Item Type: Article
Uncontrolled Keywords: AMPLIFIED RIBOSOMAL DNA; SPECIES CONCEPT; COMPLEX; cluster analysis; intersterility groups; microscopical characters; PCR; Phellinus pini group; restriction-fragment
Subjects: 500 Science > 580 Botanical sciences
Divisions: Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften
Depositing User: Dr. Gernot Deinzer
Date Deposited: 07 Nov 2023 09:59
Last Modified: 07 Nov 2023 09:59
URI: https://pred.uni-regensburg.de/id/eprint/51899

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