Will, Andreas and Tanner, Widmar (1996) Importance of the first external loop for substrate recognition as revealed by chimeric Chlorella monosaccharide H+ symporters. FEBS LETTERS, 381 (1-2). pp. 127-130. ISSN 0014-5793, 1873-3468
Full text not available from this repository.Abstract
It had been shown previously by heterologous expression in Schizosaccharomyces pombe, that the two monosaccharide/H+ symporters HUP1 and HUP2 of Chlorella kessleri differ significantly concerning their substrate specificity: HUP1 transports predominantly D-glucose while HUP2 prefers D-galactose. Several chimeric transporters were constructed and their substrate specificities determined. Surprisingly, it is sufficient to replace the first part of the external loop 1 of the HUP1 symporter by the corresponding portion of HUP2 to improve transport and also to decrease the K-m value for D-galactose, Additional data indicating the importance of the first loop for substrate recognition and binding are discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HEXOSE; COTRANSPORTER; EXPRESSION; TRANSPORT; VULGARIS; PH; sugar transport; HUP1; chimeric transporter; Chlorella kessleri; D-galactose; Schizosaccharomyces pombe |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Nov 2023 10:59 |
| Last Modified: | 07 Nov 2023 10:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51921 |
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