Kees, Frieder and Jehnich, Doris and Grobecker, Horst (1996) Simultaneous determination of acetylsalicylic acid and salicylic acid in human plasma by high-performance liquid chromatography. JOURNAL OF CHROMATOGRAPHY B, 677 (1). pp. 172-177. ISSN 0378-4347
Full text not available from this repository.Abstract
A high-performance liquid chromatographic (HPLC) method is described for the simultaneous determination of acetylsalicylic acid (ASA) and its main metabolite salicylic acid (SA) in human plasma. Acidified plasma is deproteinized with acetonitrile which is separated from the aqueous layer by adding sodium chloride. ASA and SA are extracted into the acetonitrile layer with high yield, and determined by reversed-phase HPLC (column: Novapak C-18 4 mu m silica, 150X4 mm I.D.; eluent: 740 ml water, 900 mu l 85% orthophosphoric acid, 180 ml acetonitrile) and photometric detection (237 nm). 2-Methylbenzoic acid is used as internal standard. The method allows the determination of ASA and SA in human plasma as low as 100 ng/ml with good precision (better than 10%). The assay was used to determine the pharmacokinetic parameters of ASA and SA following oral administration of 100-500 mg ASA in healthy volunteers.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BIOLOGICAL-FLUIDS; ASPIRIN; METABOLITES; URINE; acetylsalicylic acid; salicylic acid |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Nov 2023 11:08 |
| Last Modified: | 07 Nov 2023 11:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51924 |
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