Lipid analysis of Methanopyrus kandleri

Hafenbradl, Doris and Keller, Martin and Stetter, Karl O. (1996) Lipid analysis of Methanopyrus kandleri. FEMS MICROBIOLOGY LETTERS, 136 (2). pp. 199-202. ISSN 0378-1097, 1574-6968

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Abstract

Non-polar and polar lipids were isolated from Methanopyrus kandleri. Non-polar lipids accounted for 50% w/w of total lipids, with a high proportion of 2,3-di-O-geranylgeranyl-sn-glycerol, 2,3-di-O-phytanyl-sn-glycerol and geranylgeraniol. The core lipids prepared by mild acid methanolysis consisted exclusively of 2,3-di-O-phytanyl-sn-glycerol. Two-dimensional TLC showed mostly glycolipids, and minor amounts of aminophospholipids, phosphoglycolipids and phospholipids. The purification yielded three diglycosyl-lipids (50% of total polar lipids), one triglycosyl-lipid (5%) and six glycosyl-lipids with five glycosyl-groups (36%), which consisted of glucose, galactose and mannose. The lipid analysis supports the unique position of Methanopyrus kandleri within the 16S rRNA-based phylogenetic tree.

Item Type: Article
Uncontrolled Keywords: METHANOBACTERIUM-THERMOAUTOTROPHICUM; METHANOGENIC ARCHAEBACTERIA; POLAR LIPIDS; ETHER LIPIDS; 110-DEGREES-C; SQUALENES; BACTERIA; Methanopyrus kandleri; 2,3-di-O-geranylgeranyl-sn-glycerol; lipid composition; glycolipid
Subjects: 500 Science > 570 Life sciences
Divisions: Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum)
Depositing User: Dr. Gernot Deinzer
Date Deposited: 14 Nov 2023 07:13
Last Modified: 14 Nov 2023 07:13
URI: https://pred.uni-regensburg.de/id/eprint/51927

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