Wrobel, Karl-Heinz and Bickel, Daniela and Kujat, Richard (1996) Immunohistochemical study of seminiferous epithelium in adult bovine testis using monoclonal antibodies against Ki-67 protein and proliferating cell nuclear antigen (PCNA). CELL AND TISSUE RESEARCH, 283 (2). pp. 191-201. ISSN 0302-766X, 1432-0878
Full text not available from this repository.Abstract
The distribution pattern of proliferating cell nuclear antigen (PCNA) and Ki-67 protein was studied in adult bovine seminiferous epithelium by means of immunohistochemistry using monoclonal antibodies. Tailoring the methodological protocol for each of the two proliferation markers was a necessary prerequisite for obtaining optimal results in tubular sections and whole-mounts. A-, I- and B-spermatogonia displayed PCNA-positive nuclei, except during meta-, ana- and telophases of mitosis. PCNA-negative nuclei in the basal tubular compartment, excluding those from non-cycling Sertoli cells, belonged to the spermatogonia precursor cell line. However, only about 30%, 45% and 47% of the respective A-, I-, B-spermatogonia had positive nuclei after exposure to the MIB-1 antibody directed against the Ki-67 protein. Spermatogonia with MIB-1-negative nuclei represented cells in the G(1)-phase. Both antibodies reacted intensely with the nuclei of preleptotene primary spermatocytes. PCNA reactivity was also present during leptotene through pachytene. Ki-67 protein expression was absent during leptotene and zygotene but was again encountered during pachytene and meiosis I and II. Anti-PCNA/anti-protein gene product 9.5 double-labelling indicated that the transition from spermatogonia precursor cells into A(1)-spermatogonia is not strictly synchronized in a given tubular segment, a possible reason for the flexibility in A-spermatogonial propagation seen in bovine seminiferous tubules.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DNA POLYMERASE-DELTA; AUXILIARY PROTEIN; CYCLE; LOCALIZATION; SPERMATOGENESIS; ASSOCIATION; PEROXIDASE; EXPRESSION; DETECTS; BULL; testis; seminiferous epithelium; proliferating cell nuclear antigen (PCNA); Ki-67 protein; MIB-1 antibody; immunohistochemistry; tubular whole-mounts; bovine |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Nov 2023 08:40 |
| Last Modified: | 14 Nov 2023 08:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/51954 |
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