Hofmeister, R and Mannel, DN and Falk, W (1996) Evidence for an intracellular activation loop in the IL-1 system. JOURNAL OF INFLAMMATION, 47 (3). pp. 151-163. ISSN 1078-7852,
Full text not available from this repository.Abstract
The mechanism of action of the pleiotropic cytokine interleukin-1 (IL-1) is only incompletely understood. A unique feature among cytokines is its internalization and translocation to the nuclear area in nondegraded form, suggesting intracellular activities of the molecule. To define activities of that kind, a pair of IL-1 receptor type I (IL-1RI)-positive EL4 thymoma cells with differential receptor functionality was transfected with plasmids which caused intracellular expression of FLAGIL-1 alpha fusion peptides. Intracellular delivery of IL-1 costimulated expression of IL-2 mRNA and production of IL-2 protein. This effect was not mediated by the plasma membrane IL-1RI. The cells were permanently activated and in cells with functional IL-1RI, appearance of membrane IL-1RI was abrogated. Thus, intracellularly delivered IL-1 can bypass and replace the plasma membrane IL-1RI, possibly via an as yet undefined intracellular receptor. This is a new modality of IL-1 action and suggests a role for the intracellular IL-1R antagonists (icIL-1Ra). (C) 1997 Wiley-Liss, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INTERLEUKIN-1 RECEPTOR ANTAGONIST; NUCLEAR-LOCALIZATION SEQUENCE; T-CELL LINES; SIGNAL-TRANSDUCTION; GROWTH-FACTOR; MEDIATED ENDOCYTOSIS; MONOCLONAL-ANTIBODY; GENE-EXPRESSION; INTERNALIZATION; CLONING; interleukin-1; intracellular; internationalization; interleukin-1 receptor |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52068 |
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