Praast, G and Hofmeister, R and Grube, K and Hans, W and Kuhlwein, T and Krammer, PH and Falk, W (1996) The internalized interleukin-1 activation complex in fibroblasts localizes to the Golgi apparatus. JOURNAL OF INFLAMMATION, 46 (3). pp. 125-138. ISSN 1078-7852,
Full text not available from this repository.Abstract
In order to investigate binding and internalization of interleukin-1 (IL-1) by confocal laser scanning microscopy, we established a model system comprising an IL-1 receptor type I (IL-1RI) overexpressing transfectant of the murine fibrosarcoma cell line L929 (L929RI) and an N-terminal FLAG-tagged human recombinant IL-1 alpha (FLAG-IL-1 alpha). The function of the transfected receptors was shown by their IL-1-induced association with a kinase activity The biological activity of the purified FLAG-IL-1 alpha was comparable to the unmodified molecule. L929RI cells were exposed to saturating concentrations of FLAG-IL-1 alpha. Two-color fluorescence analysis revealed increasing cell surface binding of FLAG-IL-1 alpha to the receptor over 30 min. This was followed by internalization and accumulation of the ligand/receptor complex at the Golgi apparatus. After 3 hr the receptor signal significantly decreased and patches of FLAG-epitopes reappeared on the cell surface, no longer colocalized with IL-1RI. Thus in this model, the previously assumed nuclear accumulation of IL-1 was not detected but rather localization of the internalized IL-1/IL-1RI-complex to the Golgi apparatus was found. Direct effects of IL-1 on the nucleus or the nuclear membrane therefore are unlikely. (C) 1996 Wiley-Liss, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RECEPTOR-MEDIATED ENDOCYTOSIS; T-CELL LINES; NUCLEAR-LOCALIZATION; IL-1; EXPRESSION; ANTAGONIST; PROTEIN; GENE; SEQUENCE; CLONING; colocalization; confocal laser scanning; microscopy; internalization; IL-1 receptor; IL-1; signal transduction |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52069 |
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