Gahrtz, Manfred and Schmelzer, Elmon and Stolz, Jürgen and Sauer, Norbert (1996) Expression of the PmSUC1 sucrose carrier gene from Plantago major L is induced during seed development. PLANT JOURNAL, 9 (1). pp. 93-100. ISSN 0960-7412, 1365-313X
Full text not available from this repository.Abstract
A cDNA clone of the plasma membrane sucrose-Ht symporter PmSUC1 from Plantago major L. has been isolated and expressed in Saccharomyces cerevisiae. The PmSUC1 protein was characterized in transgenic yeast and in proteoliposomes with an artificial proton-motive-force (pmf) generator. PmSUC1 catalyzes the active uptake of sucrose or maltose in the presence of pmf and is sensitive to uncouplers. Unlike the extremely pH-dependent PmSUC2 sucrose-H+ symporter, PmSUC1 is relatively insensitive to changes of the extracellular pH. In leaves and petioles of P. major, expression of PmSUC1 mRNA is restricted to the vascular system. The important new feature about PmSUC1 is that the highest mRNA levels are found in non-vascular tissue of P. major flowers where the gene is transiently expressed during the early stages of seed development. In site hybridization experiments show that PmSUC1 is expressed only in young ovules; the putative physiological role of PmSUC1 is discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PLASMA-MEMBRANE VESICLES; HETEROLOGOUS EXPRESSION; CDNA CLONING; TRANSPORT; SYMPORT; COTRANSPORTER; INFECTION; LEAVES; YEAST |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Nov 2023 08:50 |
| Last Modified: | 15 Nov 2023 08:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52119 |
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