Fabry, Stefan and Beyser, Kurt (1996) YptV2p, a membrane-associated small G protein abundant during embryogenesis in the green alga Volvox carteri. PROTOPLASMA, 190 (1-2). pp. 79-87. ISSN 0033-183X
Full text not available from this repository.Abstract
The multicellular fresh-water alga Volvox carteri contains at least six small G protein-encoding genes (ypt genes) whose products are probably involved in intracellular vesicle transport. Four of them, YptV1, YptV3, YptV4, and YptV5, have been isolated and characterized previously. Here we report the cloning of yptV2 cDNA, the production of recombinant His-tagged YptV2p protein (reYptV2p) in E. coli, and the analysis of its GTPase activity and intracellular localization. YptV2p is predominantly present in dividing Volvox embryos. It is a membrane-associated protein which is localized to the cell periphery (plasma membrane or plasma-membrane-associated vesicles), probably by a lipid moiety. Purified, E. coli-expressed YptV2p binds GTP specifically, and has a typically low intrinsic GTPase activity (k(cat) = 0.004/min), which is enhanced by a GTPase activating protein activity present in Volvox. Our observations suggest a role of YptV2p in secretion, with a peak during the rapid cleavages of the Volvox embryo.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GTP-BINDING PROTEINS; ASEXUAL LIFE-CYCLE; RAS PROTEIN; PHYLOGENETIC-RELATIONSHIPS; EXPRESSION; FAMILY; GENES; RELATIVES; PRODUCT; YEAST; ypt/rab genes; GTPase-activating protein; differentiation; Ras superfamily; Chlamydomonas |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Cell Cycle Control |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Nov 2023 08:59 |
| Last Modified: | 15 Nov 2023 08:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52122 |
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