Riedel, Astrid and Fetzner, Susanne and Rampp, Martin and Lingens, Franz and Liebl, Ursula and Zimmermann, Jean-Luc and Nitschke, Wolfgang (1995) EPR, electron spin echo envelope modulation, and electron nuclear double resonance studies of the 2Fe2S centers of the 2-halobenzoate 1,2-dioxygenase from Burkholderia (Pseudomonas) cepacia 2CBS. JOURNAL OF BIOLOGICAL CHEMISTRY, 270 (52). pp. 30869-30873. ISSN 0021-9258, 1083-351X
Full text not available from this repository.Abstract
The 2-halobenzoate 1,2-dioxygenase from Burkholderia (Pseudomonas) cepacia 2CBS (Fetzner, S., Muller, R., and Lingens, F. (1992) J. Bacteriol. 174, 279-290) contains both a ferredoxin-type and a Rieske-type 2Fe2S center, These two significantly different 2Fe2S clusters were characterized with respect to their EPR spectra, electrochemical properties (Rieske-type cluster with g(z) = 2.025, g(y) = 1.91, g(x) = 1.79, g(av) = 1.91, E(m) = -125 +/- 10 mV; ferredoxin-type center with g(z) = 2.05, g(y) = 1.96, g(x) = 1.89, g(av) = 1.97, E(m) = -200 +/- 10 mV) and pH dependence thereof, X band electron spin echo envelope modulation and electron nuclear double resonance spectroscopy was applied to study the interaction of the Rieske-type center of the 2-halobenzoate 1,2-dioxygenase with N-14 and H-1 nuclei in the vicinity of the 2Fe2S cluster. The results are compared to those obtained on the Rieske protein of the cytochrome b(6)f complex (E(m) = +320 mV) and the water-soluble ferredoxin (E(m) = -430 mV) of spinach chloroplasts, as typical representatives of the g(av) = 1.91 and g(av) = 1.96 class of 2Fe2S centers, Properties common to all Rieske-type clusters and those restricted to the respective centers in bacterial oxygenases are discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | THERMUS RIESKE PROTEIN; MAIN CHAIN FOLD; PHTHALATE DIOXYGENASE; 2FE-2S CLUSTER; CYTOCHROME-B6F COMPLEX; 2.5-A RESOLUTION; N-COORDINATION; 2 HISTIDINES; FES CENTER; SPECTROSCOPY |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Nov 2023 09:56 |
| Last Modified: | 15 Nov 2023 09:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52152 |
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