de la Tour, Claire Bouthier and Kaltoum, H. and Portemer, C. and Confalonieri, F. and Huber, R. and Duguet, M. (1995) Cloning and sequencing of the gene coding for topoisomerase I from the extremely thermophilic eubacterium, Thermotoga maritima. BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1264 (3). pp. 279-283. ISSN 0167-4781
Full text not available from this repository.Abstract
A 2767 bp fragment containing a gene coding for a topoisomerase I from the extremely thermophilic eubacterium Thermotoga maritima (Tm TopA) has been cloned and sequenced. The protein is composed of 633 amino acids with a calculated molecular mass of 72 695 Da. It shares significant similarity with the topoisomerases I of mesophilic eubacteria. The highest score is obtained with Bacillus subtilis (44% identity); in particular, T. maritima and B. subtilis possess an insertion of 7-8 amino acids in the vicinity of the active site, that is absent in topoisomerases of other organisms. A specific feature of T. maritima topoisomerase I is its low cysteine content compared to its mesophilic homologs. It contains 5 cysteine residues, of which 4 could constitute a zinc finger motif. Finally, analysis of the regions flanking the gene reveals that Tm TopA is surrounded by two other ORFs, suggesting the occurrence of a polycistronic transcriptional unit.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SP-NOV REPRESENTS; ESCHERICHIA-COLI; DNA TOPOISOMERASE; REVERSE GYRASE; ORGANIZATION; DNA topoisomerase I; bacterium; thermotoga; thermophile; (T-maritima) |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Nov 2023 10:16 |
| Last Modified: | 15 Nov 2023 10:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52154 |
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