Stolz, Jürgen and Darnhofer-Demar, Brigitte and Sauer, Norbert (1995) Rapid purification of a functionally active plant sucrose carrier from transgenic yeast using a bacterial biotin acceptor domain. FEBS LETTERS, 377 (2). pp. 167-171. ISSN 1873-3468
Full text not available from this repository.Abstract
A rapid and efficient method has been used for the purification of a Plantago major sucrose carrier from Saccharomyces cerevisiae. The C-terminal fusion of a bacterial biotin acceptor domain to the carrier protein did not interfere with the targeting to the yeast plasma membrane nor with the catalytic activity of the sucrose carrier. The chimeric construct is biotinylated by yeast cells in vivo and represents the only biotinylated protein in yeast membranes. Solubilized biotinylated carrier protein binds selectively to immobilized monomeric avidin and can be eluted as pure protein with free biotin. The purified protein is functionally active and catalyzes the energy-dependent transport of sucrose into proteoliposomes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GLUCOSE TRANSPORTER; LACTOSE PERMEASE; PROTEINS; SEQUENCE; EXPRESSION; MEMBRANE; SUBUNIT; ENZYMES; biotinylation; heterologous expression; protein purification; reconstitution; sucrose transporter |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Nov 2023 10:26 |
| Last Modified: | 15 Nov 2023 10:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52158 |
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