Thermococcus alcaliphilus sp nov, a new hyperthermophilic archaeum growing on polysulfide at alkaline pH

Keller, Martin and Braun, Franz-Josef and Dirmeier, Reinhard and Hafenbradl, Doris and Burggraf, Siegfried and Rachel, Reinhard and Stetter, Karl O. (1995) Thermococcus alcaliphilus sp nov, a new hyperthermophilic archaeum growing on polysulfide at alkaline pH. ARCHIVES OF MICROBIOLOGY, 164 (6). pp. 390-395. ISSN 0302-8933, 1432-072X

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Abstract

A novel coccoid-shaped, hyperthermophilic, heterotrophic member of the archaea was isolated from a shallow marine hydrothermal system at Vulcano Island, Italy, The isolate grew between 56 and 90 degrees C with an optimum around 85 degrees C. The pH range fur growth was 6.5 to 10.5, with an optimum around 9.0. Polysulfide and elemental sulfur were reduced to H2S. Sulfur stimulated the growth rate. The isolate fermented yeast extract, peptone, meat extract, tryptone, and casein, Isovalerate, isobutyrate, propionate, acetate, CO2, NH3, and H2S (in the presence of S degrees) were detected as end products. Growth was not inhibited by H-2. Based on DNA-DNA hybridization and 16S rRNA partial sequences, the new isolate represents a new species of Thermococcus, which we named Thermococcus alcaliphilus. The type strain is isolate AEDII12 (DSM 10322).

Item Type: Article
Uncontrolled Keywords: PYROCOCCUS-FURIOSUS; SULFUR RESPIRATION; ELEMENTAL SULFUR; ARCHAEBACTERIA; GENUS; REPRESENTS; EUBACTERIA; REDUCTION; BACTERIA; GROWTH; archaea; hyperthermophiles; Thermococcus alcaliphilus; polysulfide reduction; alkaline pH
Subjects: 500 Science > 570 Life sciences
Divisions: Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel
Depositing User: Dr. Gernot Deinzer
Date Deposited: 15 Nov 2023 10:53
Last Modified: 15 Nov 2023 10:53
URI: https://pred.uni-regensburg.de/id/eprint/52170

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