MullerLadner, U and Gschwendtner, H and Benning, K and Lang, B (1995) Comparison of four immunoblot systems for the differentiation of antibodies against nuclear and cytoplasmic antigens. AKTUELLE RHEUMATOLOGIE, 20 (6). pp. 234-238. ISSN 0341-051X,
Full text not available from this repository.Abstract
Since recently, immunoblots for detection and differentiation of antibodies against nuclear antigens (ENA), other nuclear antigens and cytoplasmic antigens, blotted on nitrocellulose strips (Western blot technique) became commercially available. The aim of our analysis was to compare the specifity and sensitivity of four different immunblot systems (blots A, B, L, V). We examined 20 sera of patients with various clinically confirmed connective tissue diseases. The sera contained a total of 32 different antigens. The antibodies in the sera were defined by double immunodiffusion (Ouchterlony) test, immunofluorescence (HEp2 cells) and by comparison with WHO reference sera. Results: Blot a detected 81%, blot B 30 %, blot L 57 % and blot V 58 % of the auto-antibodies in the tested sera. In addition, we analysed the number of false positive bands. Blot A detected one, blot B 15, blot L none and blot V 10 false positive auto-antibodies. As sensitivity and specifity differ between the various blot systems, each clinical laboratory should first test the commercially available blot systems under daily conditions with standard sera. In comparison of four different blot systems, blot A was superior to the others with regard to sensitivity, specificity, quality and laboratory handling.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | AUTOIMMUNE-DISEASES; AUTOANTIBODIES; IMMUNODIFFUSION; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52213 |
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