Göpfert, Thea and Eckardt, Kai-Uwe and Gess, Bernhard and Kurtz, Armin (1995) Cobalt exerts opposite effects on erythropoietin gene expression in rat hepatocytes in vivo and in vitro. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 269 (5). R995-R1001. ISSN 0363-6119
Full text not available from this repository.Abstract
This study investigates the effects of hypoxia and of cobalt on erythropoietin (EPO) gene expression in hepatocytes in vivo and in vitro in neonatal, juvenile, and adult rats. With the use of the ribonuclease protection assay to quantify RNA, both hypoxia (0.1% CO or 9% O-2) and cobalt (60 mg/kg) elicit production of increased amounts of EPO mRNA in neonatal and juvenile rat liver in vivo. In vitro hepatocyte EPO gene expression could be reproducibly stimulated by hypoxia (3% O-2) but not by cobaltous chloride (50-150 mu M) within 2-20 h. Conversely, cobalt substantially attenuated the rise of EPO mRNA levels in response to hypoxia. This inhibitory effect of cobalt was mimicked by zinc but not by other metals. CO attenuated the rise of EPO mRNA levels in vitro in response to hypoxia; this inhibitory effect coincided with an inhibition of total RNA synthesis as determined by [H-3]uridine incorporation. The lack of specific inhibitory effects of CO and of specific stimulatory effects of cobalt on hepatocyte EPO gene expression in vitro suggests that a specific heme oxygen sensor may be less important than in hepatoma cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESSENGER-RNA LEVELS; DEPENDENT EXPRESSION; OXYGEN SENSOR; HEME PROTEIN; CELL-LINES; HYPOXIA; MODULATION; ENHANCER; TRANSCRIPTION; CULTURES; OXYGEN SENSING; CARBON MONOXIDE |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Dec 2023 07:54 |
| Last Modified: | 22 Dec 2023 07:54 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52214 |
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