Huber, Robert and Stöhr, Josef and Hohenhaus, Sabine and Rachel, Reinhard and Burggraf, Siegfried and Jannasch, Holger W. and Stetter, Karl O. (1995) Thermococcus chitonophagus sp. nov., a novel, chitin degrading, hyperthermophilic archaeum from a deep-sea hydrothermal vent environment. ARCHIVES OF MICROBIOLOGY, 164 (4). pp. 255-264. ISSN 0302-8933, 1432-072X
Full text not available from this repository.Abstract
From a hydrothermal vent site off the Mexican west coast (20 degrees 50'N, 109 degrees 06'W) at a depth of 2,600 m, a novel, hyperthermophilic, anaerobic archaeum was isolated. Cells were round to slightly irregular cocci, 1.2-2.5 pm in diameter and were motile by means of a tuft of flagella. The new isolate grew between 60 and 93 degrees C (optimum: 85 degrees C), from pH 3.5 to 9 (optimum: pH 6.7), and from 0.8 to 8% NaCl (optimum: 2%). The isolate was an obligate organotroph, using chitin, yeast extract, meat extract, and peptone for growth. Chitin was fermented to H-2, CO2, NH3, acetate, and formate. H2S was formed in the presence of sulfur. The chitinoclastic enzyme system was oxygen-stable, cell-associated, and inducible by chitin. The cell wall was composed of a surface layer of hexameric protein complexes arranged on a p6 lattice. The core lipids consisted of glycerol diphytanyl diethers and acyclic and cyclic glycerol diphytanyl tetraethers. The G+C content was 46.5 mol%. DNA/DNA hybridization and 16S rRNA sequencing indicated that the new isolate belongs to the genus Thermococcus, representing a new species, Thermococcus chitonophagus. The type strain is isolate GC74, DSM 10152.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GEN-NOV; ENZYMATIC AMPLIFICATION; PYROCOCCUS-FURIOSUS; MARINE-BACTERIA; ARCHAEBACTERIA; DEGRADATION; REPRESENTS; CLASSIFICATION; ORGANISMS; ARCHAEOBACTERIUM; THERMOCOCCUS; ARCHAEA; HYPERTHERMOPHILE; DEEP-SEA HYDROTHERMAL VENT; CHITIN; RIFTIA PACHYPTILA |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Jan 2024 09:02 |
| Last Modified: | 04 Jan 2024 09:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52278 |
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