STRAUB, RH and LANG, B and FALK, W and SCHOLMERICH, J and SINGER, EA (1995) IN-VITRO SUPERFUSION METHOD FOR THE INVESTIGATION OF NERVE-IMMUNE CELL-INTERACTION IN MURINE SPLEEN. JOURNAL OF NEUROIMMUNOLOGY, 61 (1). pp. 53-60. ISSN 0165-5728,
Full text not available from this repository.Abstract
Immune defence mechanisms can be modulated by brain function. To study such interactions, an in vitro method was developed to examine the release of cytokines and norepinephrine (NE) after electrical stimulation. Slices of mouse spleen were placed in chambers with a volume of 80 mu l and superfused with culture medium. To characterize electrically evoked NE release and cell viability a suitable stimulation protocol was developed using of [H-3]NE. As parameter for immune function, modulation of interleukin-6 (IL-6) release by the spleen cells brought about by electrical stimulation was investigated. Splenic [H-3]NE overflow was calcium-dependent, tetrodotoxin-sensitive and elicited by 54 mM potassium. Electrically evoked [H-3]NE release at 22 h was about 80% of the release at 5.3 h. Electrical stimulation substantially reduced IL-6 secretion at 6 h (control: 143.4 +/- 14.3 vs, electrical: 71.3 +/- 7.9 pg/ml/10(6) leukocytes, P = 0.0001). This effect was inhibited in a concentration-dependent manner by the beta-adrenergic antagonist propranolol (P = 0.0298, EC(50) approx. 10(-7) M). In conclusion, this new technique allows long-term. investigation of cell function in slices of murine spleen. In addition, these are the first in vitro data indicating the presence of a functional neuroimmunological link in murine lymphoid tissue.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TUMOR-NECROSIS-FACTOR; PRESYNAPTIC RECEPTOR SYSTEMS; TRANSMITTER RELEASE; BRAIN-SLICES; CYCLIC-NUCLEOTIDES; GROWTH-FACTOR; NOREPINEPHRINE; INDUCTION; INTERLEUKIN-6; PROLIFERATION; SUPERFUSION TECHNIQUE; MURINE SPLEEN SLICE; NOREPINEPHRINE RELEASE; INTERLEUKIN-6 RELEASE; NEUROIMMUNOLOGICAL LINK |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52405 |
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