RUSCHOFF, J and ELSASSER, HP and ADLER, G (1995) CORRELATION OF NUCLEOLAR ORGANIZER REGIONS WITH SECRETORY AND REGENERATIVE PROCESS IN EXPERIMENTAL CERULEIN-INDUCED PANCREATITIS IN THE RAT. PANCREAS, 11 (2). pp. 154-159. ISSN 0885-3177,
Full text not available from this repository.Abstract
Silver staining of nucleolar organizer regions (AgNORs) demonstrates loops of DNA that transcribe ribosomal RNA. Their number and size have been attributed to rDNA transcription activity involved in protein synthesis and thus associated with proliferation. The exact relationship among proliferation, protein synthesis, and expression of AgNORs is, however, not yet well established. We therefore investigated AgNORs in an experimental model of cerulein-induced rat pancreatitis. During secretory stimulation with maximal doses of cerulein (0.25 mu g/kg/h) for 12 h, AgNOR number and size per nucleus as well as H-3-thymidine label index did not change, although there was a marked increase in pancreatic volume flow, up to 150%, and of protein synthesis rate, up to 180% of the control levels. In contrast, after infusion of supramaximal doses of cerulein (5.0 mu g/kg/h), AgNOR and H-3-thymidine label values rose significantly, with a distinct peak at day 3 after induction of pancreatitis. Most interestingly, AgNOR number and size were elevated 12 h before DNA replication started as determined by H-3-thymidine incorporation. At the same time intracellular protein synthesis proved to be decreased similar to 30-50% compared to controls. Our data confirm that AgNOR is a marker of proliferation that reflects regulatory events in the cell cycle earlier than H-3-thymidine incorporation. Here we demonstrate for the first time that this phenomenon is independent of the total intracellular protein synthesis rate.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEINS; CELLS; STIMULATION; VISUALIZATION; SECRETAGOGUE; MEMBRANE; TUMORS; COUNTS; KI-67; EXPERIMENTAL RAT PANCREATITIS; PROLIFERATION; PROTEIN SYNTHESIS; AGNOR; H-3 THYMIDINE |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52415 |
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