BEAUCAMP, N and OSTENDORP, R and SCHURIG, H and JAENICKE, R (1995) CLONING, SEQUENCING, EXPRESSION AND CHARACTERIZATION OF THE GENE ENCODING THE 3-PHOSPHOGLYCERATE KINASE - TRIOSEPHOSPHATE ISOMERASE FUSIONPROTEIN FROM THERMOTOGA-MARITIMA. PROTEIN AND PEPTIDE LETTERS, 2 (1). pp. 281-286. ISSN 0929-8665,
Full text not available from this repository.Abstract
The gene encoding 3-phosphoglycerate kinase (PGK) and triosephosphate isomerase (TIM) from the hyperthermophilic bacterium Thermotoga maritima has been cloned in E. coli. The gene consists of two overlapping open reading frames. In E. coli, as in Thermotoga maritima, two proteins with corresponding N-termini are expressed from this gene: a monomeric 43 kDa protein (PGK) and a tetrameric fusionprotein (PGKfus) of 280 kDa. The fusion protein reveals PGK and TIM activity and is synthesized as the result of a (-1)- frameshift. The nucleotide sequence was confirmed and the fusion protein was expressed and characterized. The recombinant enzymes are authenthic to those in Thermotoga maritima.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COMPLETE NUCLEOTIDE-SEQUENCE; ESCHERICHIA-COLI; LACTATE-DEHYDROGENASE; FRAMESHIFT; CHAIN; RECONSTITUTION; STABILITY; VECTORS; DNA; RNA; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52418 |
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