KLOTH, S and EBENBECK, C and KUBITZA, M and SCHMIDBAUER, A and ROCKL, W and MINUTH, WW (1995) STIMULATION OF RENAL MICROVASCULAR DEVELOPMENT UNDER ORGANOTYPIC CULTURE CONDITIONS. FASEB JOURNAL, 9 (10). pp. 963-967. ISSN 0892-6638,
Full text not available from this repository.Abstract
The development of the renal vascular system requires the coordinated action of soluble morphogenic factors and specific extracellular matrix components, Despite intensive research it remains unknown whether the humoral or the environmental component is more important in the development of renal microvessels, The prolonged serum-free culture of embryonic kidney cortex explants was achieved by means of a newly developed perfusion culture system, This system made the investigation of renal vascular development under defined organotypic conditions possible, Thus, growth factors such as vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and hormones (aldosterone, vitamin D-3) could be applied without the interference with serum components. Medium supplementation with VEGF or aldosterone in combination with vitamin D-3 resulted in the coordinated proliferation of endothelial cells in the explant, A well-developed collecting duct epithelium and numerous tubular structures were always observed, In contrast, only a uniform cell layer was found between fibrous organ capsule and the collecting duct epithelium after bFGF application, but neither tubular structures nor endothelial cells. Thus, the experiments indicate that bFGF alone has no stimulating effect on the growth of the renal microvasculature under perfusion culture conditions.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENDOTHELIAL GROWTH-FACTOR; DEVELOPING KIDNEY; RABBIT KIDNEY; CELLS; DIFFERENTIATION; ANGIOGENESIS; RECEPTORS; NEPHRON; SPECIFICITY; EXPRESSION; KIDNEY; VASCULAR DEVELOPMENT; PERFUSION CELL CULTURE; ENDOTHELIUM; GROWTH FACTORS |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52450 |
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