MEUSEL, M and RENNEBERG, R and SPENER, F and SCHMITZ, G (1995) DEVELOPMENT OF A HETEROGENEOUS AMPEROMETRIC IMMUNOSENSOR FOR THE DETERMINATION OF APOLIPOPROTEIN-E IN SERUM. BIOSENSORS & BIOELECTRONICS, 10 (6-7). pp. 577-586. ISSN 0956-5663,
Full text not available from this repository.Abstract
Apolipoprotein (ape) E is a constituent of serum lipoproteins and can serve as a diagnostic parameter for the assessment of disorders of lipid metabolism. For the determination of apo E in serum a sandwich-type amperometric immunosensor using disposable membranes was developed. The best results were obtained by using a site-directed attachment of a monoclonal capture antibody to a hydrazide-functionalized membrane surface. Bound antigen was then determined with the aid of a polyclonal antibody labelled with alkaline phosphatase in conjunction with p-aminophenyl phosphate as substrate. In this approach a carbon working electrode (vs. Hg/HgCl2) was used, and enzymatically generated p-aminophenol could be monitored with a detection limit of 40 pmol and a linear range of 26-20000 nM. The sensor displayed a linear response from 50 to 1000 ng ml(-1) apo E. In contrast, antibody coupling through primary amino groups led to a total loss of antigen binding capacity in this assay configuration. The approach with site-directed immobilization however, not only allowed the determination of apo E in serum, but also the determination of antibody cross-reactivity against the apolipoproteins AI, AII and B.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ELECTROCHEMICAL ENZYME-IMMUNOASSAY; E POLYMORPHISM; IMMOBILIZATION; PROTEIN; HYPERLIPIDEMIA; ALKALINE PHOSPHATASE; AMPEROMETRIC IMMUNOSENSOR; APOLIPOPROTEIN E; CARBON ELECTRODE; GLUCOSE OXIDASE; P-AMINOPHENYL PHOSPHATE; SITE-DIRECTED IMMOBILIZATION |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52493 |
Actions (login required)
 |
View Item |
