GODT, DE and RIEGEL, A and ROITSCH, T (1995) REGULATION OF SUCROSE SYNTHASE EXPRESSION IN CHENOPODIUM-RUBRUM - CHARACTERIZATION OF SUGAR INDUCED EXPRESSION IN PHOTOAUTOTROPHIC SUSPENSION-CULTURES AND SINK TISSUE-SPECIFIC EXPRESSION IN PLANTS. JOURNAL OF PLANT PHYSIOLOGY, 146 (3). pp. 231-238. ISSN 0176-1617, 1618-1328
Full text not available from this repository.Abstract
The effect of source/sink modifications on the enzyme activity and on the steady state level of mRNA of sucrose synthase has been analyzed in Chenopodium rubrum. A sucrose synthase cDNA with high homology to sucrose synthases from both monocotyledonous and dicotyledonous plants has been cloned and used as a homologous probe for Northern blot analyses. Photoautotrophic suspension culture cells, which may be shifted to mixotrophic growth by adding sugars, have been used as a model system to investigate the transition between autotrophic and heterotrophic growth. The higher activity of sucrose synthase after preincubation in the presence of D-glucose, D-fructose or sucrose correlates with an elevated level of mRNA. The sucrose synthase gene was fully induced above a concentration of 20 mM glucose and the steady state level of mRNA was already elevated Ih after the addition of glucose. Induction of sucrose synthase by 6-desoxyglucose suggests that the non-phosphorylated glucose is the signal for the sugar induced gene expression. Both the enzyme activity and the mRNA level showed a sink tissue specific distribution in plants. The data suggest that sucrose synthase is important both for sink metabolism and the source sink transition.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE-OXYGENASE; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; METABOLIC-REGULATION; GENES; GLUCOSE; STARCH; GROWTH; MAIZE; LEAVES; TRANSCRIPTION; CHENOPODIUM RUBRUM L; SINK METABOLISM; SUCROSE SYNTHASE; SUGAR INDUCTION |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52503 |
Actions (login required)
 |
View Item |
