DELLABRUNA, R and BERNHARD, I and GESS, B and SCHRICKER, K and KURTZ, A (1995) RENIN GENE AND ANGIOTENSIN-II AT(1) RECEPTOR GENE-EXPRESSION IN THE KIDNEYS OF NORMAL AND OF 2-KIDNEY/ONE-CLIP RATS. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 430 (2). pp. 265-272. ISSN 0031-6768,
Full text not available from this repository.Abstract
This study aimed to investigate the inter-relation between the angiotensin II (ANG II) AT(1) receptor and renin gene expression in rat kidneys. To this end, renin mRNA levels and mRNA levels for AT(1a) and AT(1b) were assayed by RNase protection in the kidneys of normal rats, in animals treated with the AT(1) antagonist losartan and in rats bearing 0.2-mm left renal artery clips for 2 days. In normal rats, we found a negative correlation between renin mRNA levels and AT(1a) receptor mRNA levels. Losartan led to a fourfold increase in renin mRNA levels without changing AT(1) receptor mRNA levels. Unilateral renal artery clipping increased renin mRNA levels fourfold in the clipped kidney and suppressed renin mRNA levels in the contralateral kidneys. AT(1) receptor mRNA levels were not changed in the contralateral intact kidneys, but were significantly decreased by 15-25% in the clipped kidneys. Renin mRNA levels were inversely correlated to AT(1a) mRNA levels in the clipped, but not in the contralateral, kidneys. Our findings suggest that the systemic activity of the renin angiotensin system has no regulatory influence on renal AT(1) receptor gene expression. Renin mRNA levels in normal and in clipped kidneys appear to be negatively determined by the level of AT(1a) receptor gene expression. Thus modulation of AT(1a) receptor gene expression could be a pathway for indirect modulation of renin gene expression by ANG II. This conclusion is in agreement with the observation that AT(1) receptor antagonists are powerful stimulators of the renin system.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TYPE-1 RECEPTOR; MESSENGER-RNA; SUBTYPES; CLONING; CELLS; JUXTAGLOMERULAR CELLS; RNASE PROTECTION |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52513 |
Actions (login required)
 |
View Item |
