SCHIRMBECK, R and DEML, L and MELBER, K and WOLF, H and WAGNER, R and REIMANN, J (1995) PRIMING OF CLASS I-RESTRICTED CYTOTOXIC T-LYMPHOCYTES BY VACCINATION WITH RECOMBINANT PROTEIN ANTIGENS. VACCINE, 13 (9). pp. 857-865. ISSN 0264-410X, 1873-2518
Full text not available from this repository.Abstract
We investigated the specific priming of MHC class I-restricted cytotoxic T lymphocytes (CTL) by different protein antigen preparations in mice. The recombinant viral protein antigens tested are of potential relevance for the design of subunit vaccines. They include the hepatitis B virus (HBV) surface antigen (S-antigen), the HIV-1 gp160 envelope protein, and a chimeric HIV-1 Pr55-gag/V3-3 retrovirus-like particle. In addition, ovalbumin (OVA) was tested, The native or denatured particulate (multimeric) or monomeric form of these protein antigens was injected by various routes into mice. Class I-restricted CTL were efficiently primed by a single low-dose injection of HBV S-antigen particles or the chimeric HIV-1 Pr55-gag/V3-3 particles. After SDS-denaturation, gel-purified monomeric S-antigen and monomeric Pr55-gag/V3-3 fusion protein were still very efficient in priming CTL. CTL sensitization was not detected in a (primary or boosted) response to even high doses of native OVA or native HIV-1 gp160. Denaturation of these two antigens by detergent strikingly increased their immunogenicity for CTL. Immunization of mice with non-treated or SDS-denatured antigenic peptides representing the relevant CTL-defined epitopes of the tested protein antigens did not prime CTL. These data indicate that native, particulate and denatured, monomeric protein antigens efficiently stimulate a class I-restricted CTL response.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SYNTHETIC LIPOPEPTIDE VACCINE; VIRUS ENVELOPE GLYCOPROTEIN; SOLUBLE-PROTEIN; HEPATITIS-B; IMMUNE-RESPONSE; CELL BIOLOGY; INVIVO; INDUCTION; RECOGNITION; PARTICLES; CD8 LYMPHOCYTES; HBV-HBSAG; HIV-1 GP160; OVALBUMIN |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52523 |
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