SCHLEGEL, J and BOCKER, T and ZIRNGIBL, H and HOFSTADTER, F and RUSCHOFF, J (1995) DETECTION OF MICROSATELLITE INSTABILITY IN HUMAN COLORECTAL CARCINOMAS USING A NONRADIOACTIVE PCR-BASED SCREENING TECHNIQUE. VIRCHOWS ARCHIV-AN INTERNATIONAL JOURNAL OF PATHOLOGY, 426 (3). pp. 223-227. ISSN 0945-6317,
Full text not available from this repository.Abstract
The aim of the present study was to establish a rapid, non-radioactive screening method for the detection of microsatellite instability (MIN). MIN is the primary characteristic of the mutator phenotype in tumours constituting hereditary non-polyposis colon cancers (HNPCC). We investigated 30 patients suffering from colorectal cancer using a non-radioactive PCR-based technique. MIN was present in 7 of 30 (23%) of the cases. There was a statistically significant correlation between MIN and localization of the tumour. Five of 7 (72%) tumours with MW but only 4 of 23 (17%) tumours without MIN were localized in the proximal colon (P<0.01). There was a tendency to higher MIN frequency in tumours of patients with familial clustering of cancers. However, this was statistically not significant (P>0.05). In addition, no correlation between MIN and tumour grade and stage was found. For the investigations in the present study we used a non-radioactive PCR-based method followed by denaturating polyacrylamide gel electrophoresis and silver staining. This method is highly sensitive and reproducible. Thus, PCR-based analysis using a non-radioactive staining technique represents a comprehensive tool for MIN screening in diagnostic pathology.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DINUCLEOTIDE REPEAT POLYMORPHISM; GENOMIC INSTABILITY; CANCER; LOCUS; COLON; COLON CANCER; HEREDITARY NONPOLYPOSIS COLON CANCER; MICROSATELLITE; POLYMERASE CHAIN REACTION |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52679 |
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