Matysik, Silke and Schmitz, Gerd (2015) Determination of steroid hormones in human plasma by GC-triple quadrupole MS. STEROIDS, 99. pp. 151-154. ISSN 0039-128X, 1878-5867
Full text not available from this repository. (Request a copy)Abstract
A fast and sensitive GC-MS/MS method is proposed to determine pregnenolone, dehydroepiandrosterone (DHEA), testosterone and dihydrotestosterone from human plasma. Steroids were extracted by liquid/ liquid extraction, and derivatized with N-methyl-N-trimethylsilyl-trifluoracetamide. Electron ionization at 30 eV was applied to generate high abundant precursor ions. Specific precursor/product transitions were generated a priori for each compound of interest and these assays were then used to selectively detect and quantify these steroids. Using the selected reaction monitoring mode, detection limits in the pg/ml (sub nmol/l) range could be achieved for all four steroid compounds. The method was validated for imprecision, and recovery and applied in a pilot study in patients with hypercholesterolemia. Associations of increased cholesterol biosynthesis and low testosterone and dihydrotestosterone concentrations were found. (C) 2015 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM MASS-SPECTROMETRY; PITUITARY-TESTICULAR AXIS; MIDDLE-AGED MEN; DEHYDROEPIANDROSTERONE-SULFATE; METABOLIC SYNDROME; TESTOSTERONE DEFICIENCY; INSULIN-RESISTANCE; HEALTHY-MEN; SERUM; DISEASE; DHEA; Pregnenolone; Testosterone; Dihydrotestosterone; Steroid hormones |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Jul 2019 13:39 |
| Last Modified: | 08 Jul 2019 13:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5269 |
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