HUBER, J and HELL, W and WOLF, H (1995) A NONRADIOACTIVE MULTIPRIME SEQUENCING METHOD FOR HIV GENOMES. JOURNAL OF VIROLOGICAL METHODS, 51 (2-3). pp. 259-266. ISSN 0166-0934,
Full text not available from this repository.Abstract
A manual non-radioactive DNA sequencing protocol was developed for rapid analysis of variable HTV-1 genomes. Sets of up to ten primers were used in one sequencing reaction. After polyacrylamide gel electrophoresis and blotting onto nylon membranes the individual sequences were detected by hybridization with digoxigenin-labelled oligonucleotides and chemiluminescence. The method is applicable to any sequencing project where numerous variants of DNA fragments of several 1000 bp of length are to be analysed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DNA; ELECTROPHORESIS; POLYMERASE; PROTOCOL; PROBES; VIRUS; HIV; VARIATION OF GENOMES; MULTIPRIME SEQUENCING; CHEMILUMINESCENCE |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52768 |
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