Reuschel, Edith and Barabas, Sascha and Zeman, Florian and Bendfeldt, Hanna and Rascle, Anne and Deml, Ludwig and Seelbach-Goebel, Birgit (2017) Functional impairment of CMV-reactive cellular immunity during pregnancy. JOURNAL OF MEDICAL VIROLOGY, 89 (2). pp. 324-331. ISSN 0146-6615, 1096-9071
Full text not available from this repository. (Request a copy)Abstract
Cytomegalovirus (CMV) is the most common congenital viral infection. Mother-to-child transmission can cause severe child disability. Intact CMV-specific cell-mediated immunity (CMI) was shown to prevent uncontrolled replication in healthy individuals. This study aimed to determine whether CMV-specific CMI is impaired in pregnant women, thus potentially increasing the overall risk for active CMV replication and transmission. CMV-specific CMI in peripheral blood of 60 pregnant women was determined using T-Track (R) CMV for detection of IE-1 and pp65-reactive effector cells by IFN- ELISpot, and compared to the CMV-IgG and -IgM serostatus. CMV-specific CMI was detected in 65% of CMV-seropositive pregnant women. Five percent of CMV-IgG seronegative women showed IE-1- but not pp65-reactive cells. The overall number of CMV-reactive cells in pregnant women was significantly lower compared to a matched non-pregnant control group (P<0.001). No significant difference in CMV-specific CMI was detected in the course of the three trimesters of pregnancy of CMV-IgG seropositive women. Postpartum (median days postnatal=123), IE-1- and pp65-specific CMI remained significantly lower than in the non-pregnant control group (P<0.001 and 0.0032, respectively). Functional analysis of CMV-reactive immune cells using T-Track (R) CMV therefore suggests a systemic down-regulation of CMV-specific CMI in pregnant women. Further studies are needed to investigate whether this may be indicative of a higher susceptibility to CMV reactivation or transmission. J. Med. Virol. 89:324-331, 2017. (c) 2016 Wiley Periodicals, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CONGENITAL CYTOMEGALOVIRUS-INFECTION; NATURAL-KILLER-CELL; MEDIATED-IMMUNITY; T-CELLS; TRANSPLANT RECIPIENTS; QUANTITATIVE-ANALYSIS; PROTECTION; RESPONSES; DISEASE; VIRUS; cytomegalovirus; pregnancy; cell-mediated immunity; interferon (IFN)-gamma-enzyme-assay ELISpot |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Klinische Studien |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:01 |
| Last Modified: | 12 Feb 2019 09:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/528 |
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