RITTHALER, T and SCHOLZ, H and ACKERMANN, M and RIEGGER, G and KURTZ, A and KRAMER, BK (1995) EFFECTS OF ENDOTHELINS ON RENIN SECRETION FROM ISOLATED MOUSE RENAL JUXTAGLOMERULAR CELLS. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY, 268 (1). F39-F45. ISSN 0363-6127,
Full text not available from this repository.Abstract
This study was performed to examine the effects of endothelin (ET)-1, ET-2, and ET-3 on renin secretion from cultured mouse renal juxtaglomerular (JG) cells. Although different ETs had no consistent effect on basal renin secretion, they equipotently inhibited adenosine 3',5'-cyclic monophosphate (cAMP)-stimulated renin release with a concentration of similar to 3 nM inhibiting 50% of maximal response. ETs did not significantly affect renin release stimulated by the nitric oxide donor sodium nitroprusside (100 mu M) or that stimulated by low [2 mM ethylene glycol-bis(P-aminoethyl ether)-N,N,N',N'-tetraacetic acid] or high (3 mM CaCl2) extracellular calcium. The inhibitory effect of ETs on cAMP-dependent renin secretion was abolished by lowering extracellular calcium concentration to the nanomolar range. However, the action of ETs was not changed by the ET(A) receptor antagonist BQ-123 (100 nM) and was mimicked by ET(B) receptor agonists IRL-1620 (1 mu M), sarafotoxin S6b (1 mu M), and [Ala(1,3,11,15)]ET-1 (1 mu M). All ETs induced calcium oscillations in JG cells that were dependent on extracellular calcium and were associated with prominent calcium-activated chloride currents. These findings suggest that ETs inhibit rather selectively the cAMP-activated pathway of renin secretion through a calcium-sensitive process. The action of ETs on renal JG cells appears to be mediated via ET(B) receptors and is presumably related to activation of phospholipase C and subsequent events.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INTRACELLULAR CALCIUM; RELEASE; RECEPTOR; INHIBITION; POTENT; ENDOTHELIN RECEPTORS; CALCIUM; ADENOSINE 3',5'-CYCLIC MONOPHOSPHATE; CHLORIDE CHANNELS |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52804 |
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