BAUMANN, O and LAUTENSCHLAGER, B (1994) THE ROLE OF ACTIN-FILAMENTS IN THE ORGANIZATION OF THE ENDOPLASMIC-RETICULUM IN HONEYBEE PHOTORECEPTOR CELLS. CELL AND TISSUE RESEARCH, 278 (3). pp. 419-432. ISSN 0302-766X,
Full text not available from this repository.Abstract
Close to the bases of the photoreceptive microvilli, arthropod photoreceptors contain a dense network of endoplasmic reticulum that is involved in the regulation of the intracellular calcium concentration, and in the biogenesis of the photoreceptive membrane. Here, we examine the role of the cytoskeleton in organizing this submicrovillar endoplasmic reticulum in honeybee photoreceptors. Immunofluorescence microscopy of taxol-stabilized specimens, and electron-microscopic examination of high-pressure frozen, freeze-substituted retinae demonstrate that the submicrovillar cytoplasm lacks microtubules. The submicrovillar region contains a conspicuous F-actin system that codistributes with the submicrovillar endoplasmic reticulum. Incubation of retinal tissue with cytochalasin B leads to depolymerization of the submicrovillar F-actin system, and to disorganization and disintegration of the submicrovillar endoplasmic reticulum, indicating that an intact F-actin cytoskeleton is required to maintain the architecture of this domain of the endoplasmic reticulum. We have also developed a permeabilized cell model in order to study the physiological requirements for the interaction of the endoplasmic reticulum with actin filaments. The association of submicrovillar endoplasmic reticulum with actin filaments appears to be independent of ATP, Ca2+ and Mg2+ suggesting a tight static anchorage.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DROSOPHILA-NINAC KINASE; GLIAL-CELLS; STRUCTURAL INTERACTIONS; PURKINJE NEURONS; CALCIUM-IONS; BEE DRONE; MYOSIN-I; MEMBRANE; LOCALIZATION; BINDING; PHOTORECEPTOR CELLS; ENDOPLASMIC RETICULUM, SMOOTH; CYTOSKELETON; ACTIN FILAMENTS; MICROTUBULES; POLARITY; CALCIUM IONS; APIS MELLIFERA (INSECTA) |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52934 |
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