MALDENER, I and FIEDLER, G and ERNST, A and FERNANDEZPINAS, F and WOLK, CP (1994) CHARACTERIZATION OF DEVA, A GENE REQUIRED FOR THE MATURATION OF PROHETEROCYSTS IN THE CYANOBACTERIUM ANABAENA SP STRAIN PCC-7120. JOURNAL OF BACTERIOLOGY, 176 (24). pp. 7543-7549. ISSN 0021-9193, 1098-5530
Full text not available from this repository.Abstract
Mutant M7, obtained by transposon mutagenesis of the cyanobacterium Anabaena sp. strain PCC 7120, is impaired in the development of mature heterocysts. Under aerobic conditions, the mutant is unable to fix N-2 because of a deficiency of at least mo components of the oxygen-protective mechanisms: a hemoprotein-coupled oxidative reaction and heterocyst specific glycolipids. DNA contiguous with the inserted transposon was recovered from the mutant and sequenced. The transposon had inserted itself within a 732-bp open reading frame designated devA. The wild-type form of devA, obtained from a lambda-EMBL3 library of Anabaena sp. DNA, had the identical sequence. Directed mutagenesis of devA in the wild-type strain showed that the phenotype of the mutant was caused by insertion of the transposon. The wild-type form of devA on a shuttle vector complemented the mutation in M7. Expression of devA by whole filaments, monitored following nitrogen stepdown by using luxAB as the reporter, increased ca. eightfold during differentiation; the increase within differentiating cells was much greater. The deduced sequence of the DevA protein shows strong similarity to the ATP-binding subunit of binding protein-dependent transport systems. The product of devA may, therefore, be a component of a periplasmic permease that is required for the transition from a proheterocyst to a mature, nitrogen-fixing heterocyst.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ESCHERICHIA-COLI; HETEROCYST DEVELOPMENT; SALMONELLA-TYPHIMURIUM; PERIPLASMIC TRANSPORT; CELL-ENVELOPE; EXPRESSION; SEQUENCE; CLONING; OPERON; DIFFERENTIATION; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/52943 |
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