Rietkoetter, Eva and Bleckmann, Annalen and Bayerlova, Michaela and Menck, Kerstin and Chuang, Han-Ning and Wenske, Britta and Schwartz, Hila and Erez, Neta and Binder, Claudia and Hanisch, Uwe-Karsten and Pukrop, Tobias (2015) Anti-CSF-1 treatment is effective to prevent carcinoma invasion induced by monocyte-derived cells but scarcely by microglia. ONCOTARGET, 6 (17). pp. 15482-15493. ISSN 1949-2553,
Full text not available from this repository. (Request a copy)Abstract
The mononuclear phagocytic system is categorized in three major groups: monocyte-derived cells (MCs), dendritic cells and resident macrophages. During breast cancer progression the colony stimulating factor 1 (CSF-1) can reprogram MCs into tumor-promoting macrophages in the primary tumor. However, the effect of CSF-1 during colonization of the brain parenchyma is largely unknown. Thus, we analyzed the outcome of anti-CSF-1 treatment on the resident macrophage population of the brain, the microglia, in comparison to MCs, alone and in different in vitro co-culture models. Our results underline the addiction of MCs to CSF-1 while surprisingly, microglia were not affected. Furthermore, in contrast to the brain, the bone marrow did not express the alternative ligand, IL-34. Yet treatment with IL-34 and co-culture with carcinoma cells partially rescued the anti-CSF-1 effects on MCs. Further, MC-induced invasion was significantly reduced by anti-CSF-1 treatment while microglia-induced invasion was reduced to a lower extend. Moreover, analysis of lung and breast cancer brain metastasis revealed significant differences of CSF-1 and CSF-1R expression. Taken together, our findings demonstrate not only differences of anti-CSF-1 treatment on MCs and microglia but also in the CSF-1 receptor and ligand expression in brain and bone marrow as well as in brain metastasis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BREAST-CANCER; BRAIN METASTASIS; GROWTH-FACTOR; MACROPHAGES; PROGRESSION; PROMOTE; TISSUE; TUMORS; MICE; INHIBITION; anti-CSF-1; colonization; monocyte-derived cells; microglia; metastasis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Jul 2019 13:41 |
| Last Modified: | 10 Jul 2019 13:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5305 |
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