BURGGRAF, S and MAYER, T and AMANN, R and SCHADHAUSER, S and WOESE, CR and STETTER, KO (1994) IDENTIFYING MEMBERS OF THE DOMAIN ARCHAEA WITH RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 60 (9). pp. 3112-3119. ISSN 0099-2240,
Full text not available from this repository.Abstract
Two 16S rRNA-targeted oligonucleotide probes were designed for the archaeal kingdoms Euryarchaeota and Crenarchaeota. Probe specificities were evaluated by nonradioactive dot blot hybridization against selected reference organisms. The successful application of fluorescent-probe derivatives for whole- cell hybridization required organism-specific optimizations of fixation and hybridization conditions to assure probe penetration and morphological integrity of the cells. The probes allowed preliminary grouping of three new hyperthermophilic isolates. Together with other group-specific rRNA-targeted oligonucleotide probes, these probes will facilitate rapid in situ monitoring of the populations present in hydrothermal systems and support cultivation attempts.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SP-NOV REPRESENTS; RIBOSOMAL-RNA SEQUENCES; GEN-NOV; REDUCING ARCHAEBACTERIA; THERMOPHILIC EUBACTERIA; MICROBIAL-POPULATIONS; METHANOPYRUS-KANDLERI; PHYLOGENETIC ANALYSIS; OXIDIZING BACTERIA; AQUIFEX-PYROPHILUS; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53091 |
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