SCHMALZ, G and SCHWEIKL, H and EIBL, M (1994) GROWTH-KINETICS OF FIBROBLASTS ON BOVINE DENTIN. JOURNAL OF ENDODONTICS, 20 (9). pp. 453-456. ISSN 0099-2399,
Full text not available from this repository.Abstract
In vitro dentin barrier tests published so far show several shortcomings. Therefore, we designed a new test method using bovine dentin disks on which cells were grown on one side. In this study, we evaluated the growth kinetics of L-929 mouse fibroblasts on dentin disks of 0.75-mm thickness and 4-mm diameter derived from lower incisors of freshly slaughtered bovines. The application of 50% citric acid for 30 s was optimal for the gentle removal of the smear layer from the cut dentin slices. Growth kinetics of mouse fibroblasts depended on the initially plated cell density: optimal cell growth on the disks was observed with similar to 200 cells/mm(2), which was the same as the growth rate on the bottom of a cell culture vessel. Consequently, bovine dentin disks are a suitable substrate for normal cell growth, and this set-up can be used in a dentin barrier test.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RESTORATIVE MATERIALS; CYTO-TOXICITY; INVITRO; PULP; PERMEABILITY; IONOMER; LIGHT; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53105 |
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