OPEKAROVA, M and TANNER, W (1994) NYSTATIN CHANGES THE PROPERTIES OF TRANSPORTERS FOR ARGININE AND SUGARS - AN IN-VITRO STUDY. FEBS LETTERS, 350 (1). pp. 46-50. ISSN 0014-5793, 1873-3468
Full text not available from this repository.Abstract
In ergosterol-containing energized yeast plasma membrane vesicles nystatin (5-10 mu g/mg total lipid) caused a massive efflux of pre-accumulated arginine while the membrane potential (the principal driving force; -110 mV) decreased by only 10-30 mV. Neither the substrate fluxes nor the membrane potential was influenced by nystatin when the permease was reconstituted in ergosterol-free phospholipid vesicles. The same effect of nystatin was found with the reconstituted sugar transporter from Chlorella kessleri. It is suggested that nystatin binding to ergosterol in the vicinity of the permease releases the transport protein from its coupling to energy and converts it to a facilitator.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE; MEMBRANE; PROTEIN; BINDING; ACID; NYSTATIN; YEAST PLASMA MEMBRANE VESICLES; ARGININE TRANSPORT |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53144 |
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