KUNOW, J and LINDER, D and STETTER, KO and THAUER, RK (1994) F420H2-QUINONE OXIDOREDUCTASE FROM ARCHAEOGLOBUS-FULGIDUS - CHARACTERIZATION OF A MEMBRANE-BOUND MULTISUBUNIT COMPLEX CONTAINING FAD AND IRON-SULFUR CLUSTERS. EUROPEAN JOURNAL OF BIOCHEMISTRY, 223 (2). pp. 503-511. ISSN 0014-2956,
Full text not available from this repository.Abstract
Archaeoglobus fulgidus, a hyperthermophilic sulfate-reducing archaeon, was found to contain a membrane-bound F420H2:quinone oxidoreductase complex presumed to be involved in energy conservation during growth on lactate plus sulfate. After solubilization with dodecyl-beta-D-maltoside the complex was purified 32-fold with a yield of 24%. Using both gel filtration and native PAGE, an apparent molecular mass of approximately 270 kDa was determined. SDS/PAGE revealed the presence of at least seven polypeptides with apparent molecular masses 56, 45, 41, 39, 37, 33, and 32 kDa. The purified complex contained 1.6 mol FAD, 9 mol non-heme iron and 7 mol acid-labile sulfur/mol complex. It did not contain cytochromes, which were, however, present in the membrane fraction of A. fulgidus (3 nmol/mg membrane protein). The purified F420H2:quinone oxidoreductase complex catalyzed the reduction of 2,3-dimethyl-1,4-naphthoquinone (apparent K-m 190 mu M) with reduced coenzyme F-420 (apparent K-m 50 mu M) exhibiting a specific activity of 500 U/mg (apparent V-max) at pH 8.0 (pH optimum) and 65 degrees C (temperature optimum). 2-Methyl-1,4-naphthequinone (menadione), 2-hydroxy-1,4-naphthoquinone, 1,4-naphthoquinone, 2,3-dimethoxy-5-methyl-1,4-benzoquinone, and 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone (decyl-ubiquinone) were also reduced with F420H2, albeit with lower rates. The physiological electron acceptor of the F420H2:quinone oxidoreductase complex is most likely the menaquinone found in the membrane fraction of A. fulgidus.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SULFATE-REDUCING ARCHAEBACTERIUM; THERMOAUTOTROPHICUM STRAIN MARBURG; AEROBIC RESPIRATORY-CHAIN; THERMUS-THERMOPHILUS HB-8; METHANOGENIC BACTERIA; ESCHERICHIA-COLI; METHANOBACTERIUM-THERMOAUTOTROPHICUM; UBIQUINONE OXIDOREDUCTASE; TETRAHYDROMETHANOPTERIN FORMYLTRANSFERASE; HETERODISULFIDE REDUCTASE; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53194 |
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