Perras, Alexandra K. and Daum, Bertram and Ziegler, Christine and Takahashi, Lynelle K. and Ahmed, Musahid and Wanner, Gerhard and Klingl, Andreas and Leitinger, Gerd and Kolb-Lenz, Dagmar and Gribaldo, Simonetta and Auerbach, Anna and Mora, Maximilian and Probst, Alexander J. and Bellack, Annett and Moissl-Eichinger, Christine (2015) S-layers at second glance? Altiarchaeal grappling hooks (hami) resemble archaeal S-layer proteins in structure and sequence. FRONTIERS IN MICROBIOLOGY, 6: 543. ISSN 1664-302X,
Full text not available from this repository. (Request a copy)Abstract
The uncultivated "Candidatus Altiarchaeum hamiconexum" (formerly known as SM1 Euryarchaeon) carries highly specialized nano-grappling hooks ("hami") on its cell surface. Until now little is known about the major protein forming these structured fibrous cell surface appendages, the genes involved or membrane anchoring of these filaments. These aspects were analyzed in depth in this study using environmental transcriptomics combined with imaging methods. Since a laboratory culture of this archaeon is not yet available, natural biofilm samples with high Ca. A. hamiconexum abundance were used for the entire analyses. The filamentous surface appendages spanned both membranes of the cell, which are composed of glycosyl-archaeol. The hami consisted of multiple copies of the same protein, the corresponding gene of which was identified via metagenome-mapped transcriptome analysis. The hamus subunit proteins, which are likely to self-assemble due to their predicted beta sheet topology, revealed no similiarity to known microbial flagella-, archaella-, fimbriae- or pili-proteins, but a high similarity to known S-layer proteins of the archaeal domain at their N-terminal region (44-47% identity). Our results provide new insights into the structure of the unique hami and their major protein and indicate their divergent evolution with S-layer proteins.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SPIDER SILK PROTEINS; II SECRETION SYSTEM; SURFACE-LAYER; INDUSTRIAL APPLICATIONS; DRAGLINE SILK; IV PILI; RECOGNITION; BACTERIAL; SUBSURFACE; BIOFILM; archaea; S-layers; archaeal cell surface appendages; hami; nano-grappling hooks; double-membrane; environmental transcriptomics; electron cryo-tomography |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Jul 2019 09:46 |
| Last Modified: | 11 Jul 2019 09:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/5324 |
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