SCHIEDLMEIER, B and SCHMITT, R and MULLER, W and KIRK, MM and GRUBER, H and MAGES, W and KIRK, DL (1994) NUCLEAR TRANSFORMATION OF VOLVOX-CARTERI. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 91 (11). pp. 5080-5084. ISSN 0027-8424,
Full text not available from this repository.Abstract
Stable nuclear transformation of Volvox carteri was achieved using the cloned V. carteri nitA(+) gene (which encodes nitrate reductase) to complement a nitA(-) mutation. Following bombardment of mutant cells with plasmid-coated gold particles, putative transformants able to utilize nitrate as a nitrogen source were recovered with an efficiency of approximate to 2.5 x 10(-5). DNA analysis indicated that the plasmid integrated into the genome, often in multiple copies, at sites other than the nitA locus. Cotransformants were recovered with a frequency of 40-80% when cells were cobombarded with a selected and an unselected marker. Thus, V. carteri becomes one of the simplest multicellular organisms that is accessible to detailed molecular studies of genes regulating cellular differentiation and morphogenesis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHLAMYDOMONAS-REINHARDTII; NITRATE REDUCTASE; CELLULAR-DIFFERENTIATION; GENE; PATTERNS; COTRANSFORMATION; GREEN ALGAE; GOLD PARTICLES; HELIUM-FLOW PARTICLE GUN; NONHUMOLOGOUS RECOMBINATION |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53277 |
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