MEYER, T and KOOP, R and VONANGERER, E and SCHONENBERGER, H and HOLLER, E (1994) A RAPID LUCIFERASE TRANSFECTION ASSAY FOR TRANSCRIPTION ACTIVATION EFFECTS AND STABILITY CONTROL OF ESTROGENIC DRUGS IN CELL-CULTURES. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 120 (6). pp. 359-364. ISSN 0171-5216,
Full text not available from this repository.Abstract
A rapid assay system for measuring the potential of estrogenic drugs is introduced. Luciferase induction could be measured in estrogen-receptor-positive human MCF-7 breast cancer cells, which had been transfected with a novel luciferase reporter plasmid ERE luc. The minimal requirement was 1 h exposure to the inducing drug and 3.5 h of incubation after removal of the drug. The assay system was used to measure the stability of the drug diaqua-[1,2-bis (2,6-dichloro-4-hydroxyphenyl) ethylenediamine] platinum(II) sulfate, containing an estrogenic ligand and reactive platinum. Luciferase activity was observed only when the drug was in the culture medium and cells for short times, whereas the estrogenic ligand alone remained active. It is assumed that binding of the platinum moiety to macromolecular constituents of the culture or cells renders the drug inaccessible for binding to the estrogen receptor.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DEPENDENT MAMMARY-CARCINOMA; ANTI-ESTROGENS; CISPLATIN; COMPLEXES; RAT; ESTROGEN RECEPTOR; ESTROGENIC PLATINUM(II) COMPLEX; LUCIFERASE; TRANSFECTION; DRUG STABILITY |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53360 |
Actions (login required)
 |
View Item |
