FAGGIONI, R and GATTI, S and DEMITRI, MT and DELGADO, R and ECHTENACHER, B and GNOCCHI, P and HEREMANS, H and GHEZZI, P (1994) ROLE OF XANTHINE-OXIDASE AND REACTIVE OXYGEN INTERMEDIATES IN LPS-INDUCED AND TNF-INDUCED PULMONARY-EDEMA. JOURNAL OF LABORATORY AND CLINICAL MEDICINE, 123 (3). pp. 394-399. ISSN 0022-2143,
Full text not available from this repository.Abstract
We studied the role of reactive oxygen intermediates (ROI) in lipopolysaccharide (LPS)-induced pulmonary edema. LPS treatment (600 mu g/mouse, IP) was associated with a marked induction of the superoxide-generating enzyme xanthine oxidase (XO) in serum and lung. Pretreatment with the antioxidant N-acetylcysteine (NAC)-1 gm/kg orally, 45 minutes before LPS-or with the XO inhibitor allopurinol (AP)-50 mg/kg orally at -1 hour and +3 hours-was protective. On the other hand nonsteroidal antiinflammatory drugs (ibuprofen, indomethacin, and nordihydroguaiaretic acid) were ineffective. These data suggested that XO might be involved in the induction of pulmonary damage by LPS. However, treatment with the interferon inducer polyriboinosylic-polyribocytidylic acid, although inducing XO to the same extent as LPS, did not cause any pulmonary edema, indicating that XO is not sufficient for this toxicity of LPS. To define the possible role of cytokines, we studied the effect of direct administration of LPS (600 mu g/mouse, IP), tumor necrosis factor (TNF, 2.5 or 50 mu g/ mouse, IV), interleukin-1 (IL-1 beta, 2.5 mu g/mouse, IV), interferon-gamma (IFN-gamma, 2.5 mu g/mouse, IV), or their combination at 2.5 mu g each. In addition to LPS, only TNF at the highest dose induced pulmonary edema 24 hours later. LPS-induced pulmonary edema was partially inhibited by anti-IFN-gamma antibodies but not by anti-TNF antibodies, anti-IL-1 beta antibodies, or IL-1 receptor antagonist (IL-1Ra).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TUMOR-NECROSIS-FACTOR; RESPIRATORY-DISTRESS SYNDROME; LUNG INJURY; N-ACETYLCYSTEINE; SEPTIC SHOCK; ENDOTHELIAL-CELLS; INTERFERON-GAMMA; ENDOTOXIN; INVITRO; MICE; |
| Depositing User: | Dr. Gernot Deinzer |
| Last Modified: | 19 Oct 2022 08:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/53436 |
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